ACCUREX LDL 40 + HDL 80: Infinite
Buy Autozyme Calcium 200 Diagnostic Reagent from Accurex (CA-2). Alkaline Phosphatase reagent is used for determination of Alkaline Phosphate activity based on kinetic method using p-nitrophenyl phosphate (p-NPP).
Alkaline Phosphatase is a single reagent system using one step procedure & Alkaline Phosphatase activity can be determined in just 2.5 minutes & is is a Highly stable reagent. Alkaline Phosphatase cleaves p-nitrophenyl phosphate p- NPP in the presence of magnesium ions to form p nitrophenol & phosphate whereas, Infinite HDL-C is a reagent set for direct quantitative determination of HDL-Cholesterol in human serum or plasma based on Direct Homogenous method.
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Description
- Infinite LDL-C is a reagent set for direct quantitative determination of LDL-Cholesterol in human serum or plasma based on Direct Homogenous method.
- Direct measurement by infinite LDL-C overcomes the limitation of the Friedewald formula thereby allowing accurate determination of LDL-C.
- Infinite LDL-C is a ready-to-use, two liquid reagent system stable till expiry.
- Infinite LDL-C is linear upto 450 mg/dl.
- There is no significant interference in hyper-lipemic and hyperimmunoglobulin samples.
- In the first step, HDL, VLOL & Chylomicrons are eliminated under specific conditions.
In the second step, special reagents break up LDL and make LDL Cholesterol available for measurement. - Infinite HDL-C is a reagent set for direct quantitative determination of HDL-Cholesterol in human serum or plasma based on Direct Homogenous method.
– Direct measurement by Infinite HDL-C overcomes the limitation of precipitation method thereby allowing accurate determination of HDL-C.
– Infinite HDL-C is a ready-to-use, two liquid reagent system stable till expiry.
– Infinite HDL-C is linear upto 185 mg/dl.
– There is no significant interference in hyper-lipemic and hyper immunoglobulin samples
– Results obtained with Infinite HDL-C show excellent correlation with an ultracentrifugation method.
In the first step, LDL, VLDL & Chylomicrons are eliminated under specific conditions. Mix and incubate for 5 minutes at 37°C. Measure the absorbance A1 at 578 nm.
Reaction type ……… End-Point (2 Step)
Reaction time ………………5 + 5 mins
Wavelength……………. 546 nm.
Flow Cell temperature……………….. 37°C
Zero setting with ……………………… Distilled water
Sample volume ……………. Q.01 ml (10 µt)
Reagent volume 0.750 ml + 0.250 ml
Linearity ………….. .. 450 mg/dl
The reagent kit should be stored at 2 – 8°C and is stable till the expiry date indicated on the label. Reconstituted calibrator is stable for 3 days at 2′ – 8°C. Reconstitution stability of the calibrator may be extended upto 30 days by aliquoting and freezing the reconstituted calibrator preparation at less than -20°C.
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