ACCUREX Triglycerides 125

Glycerol released from hydrolysis of triglycerides by lipoprotein lipase is Converted by glycerol kinase into glycerol- 3- phosphate which is oxidized by glycerol phosphate oxidase to dihydroxyacetone phosphate and hydrogen peroxide. In presence of peroxidase, hydrogen peroxide oxidizes phenolic chromogen to a red coloured compound.

Reaction type ………… ………. End-Point
Reaction time …. 10 mins. at 37°C/20 mins. at R.T. (25-30°C)
Wavelength ……….. ………… 510 nm. (500 – 530 nm.)
Zero setting with ………………….. Reagent Blank
Blank absorbance limit ………… < 0.300 Abs.
Sample volume …………………………… 0.01 ml (10 jul)
Reagent volume ……….. …………. 1.0 ml
Standard concentration .. …………… 200 mg%
Linearity ………………… 800 mg/dl

The kit should be stored at 2- 8°C and is stable till the expiry date indicated on the label.
The reagent and standard are ready-to-use and are stable till expiry, when stored at 2-8°C. DO NOT FREEZE THE REAGENT.
The reagent should be stored only in the amber bottle provided to protect it from direct light. Before use swirl in the reagent gently. DO NOT SHAKE VIGOROUSLY.
Over time, the reagent may develop a light pink color. This is expected and does not affect the reagent performance. Discard the reagent if the absorbance of the same exceeds 0.300O.D. against distilled water at 510 nm.
Contamination of the reagent should be strictly avoided. Should the
reagents develop turbidity and discard the reagent.
Over time, the reagent may develop a light pink color. This is expected and does not affect the reagent performance. Discard the reagent if the absorbance of the same exceeds 0.300O.D. against distilled water at 510 nm.
Contamination of the reagent should be strictly avoided. Should the
reagents develop turbidity and discard the reagent.